HIV infection eliminated in 'humanised' mice

Temple researchers first to remove HIV-1 infection from live animals

Gene editing strategy eliminates HIV-1 infection in live animals, Temple researchers show

Researchers from Temple University and Pittsburgh University encountered several complications, including early animal experiments that began well but were unsuccessful later in the process.

"Our new study is more comprehensive", Dr. Hu said. The replication of the deadly virus can be shut down, and it can even be removed from the genetic material of infected cells by a simple gene editing strategy.

Firstly, HIV-1 was genetically inactivated in transgenic mice: this lowered the amount of RNA expression of the genes of the virus by as much as 95%.

They found the excision efficiency of their strategy reached 96 per cent in EcoHIV mice, giving the first evidence for HIV-1 eradication by prophylactic treatment with the CRISPR/Cas9 system.

In the previous study, the researchers reportedly used transgenic rat and mouse models with HIV-1 DNA incorporated into the genome of every tissue of their bodies. "These animals carry latent HIV in the genomes of human T cells, where the virus can escape detection", Dr. Hu explained. Scientists are always trying to find a method to get rid of this problem completely, but they have not been able to find an effective solution.

In all three animal models, the researchers utilized a recombinant adeno-associated viral (rAAV) vector delivery system based on a subtype known as AAV-DJ/8.

Scientists say they've used a gene-editing technique to cure mice of HIV.

The new work, led by Dr Wenhui Hu at LKSOM, builds on the same team's previous research, in which they managed to delete HIV-1 from the genome of most tissues. "The imaging system, developed by Dr".

In the first, they infected mice with HIV-1.

In a breakthrough described as a "major step" towards a permanent cure for HIV infection, living mice have been "cured" of the virus by gene therapy.

New York, May 2 Scientists have successfully removed HIV DNA from the genomes of animals - including a humanised mice - using a powerful gene-editing tool, an advance that may help eliminate the virus in humans in future. However, researchers suggested that their work which included clinical trials of the method in a human being was, by far, a "significant step".

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